CD33 抗体
Quick Overview for CD33 抗体 (ABIN118637)
抗原
See all CD33 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes the CD33 cell surface glycoprotein, a 67 kDa molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography on Protein G.
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免疫原
- Human AML cells
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亚型
- IgG1
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应用备注
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Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections (1/50-1/100): Overnight incubation recommended. Positive control tissue: lymphatic tissue. Not suitable on Paraffin Embedded Sections. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD33
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别名
- CD33 / SIGLEC3
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背景
- CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
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基因ID
- 945
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NCBI登录号
- NP_001763
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UniProt
- P20138
抗原
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