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CD33 抗体

This anti-CD33 antibody is a 小鼠 单克隆 antibody detecting CD33 in FACS, WB, IP 和 IHC (fro). Suitable for 人.
产品编号 ABIN118637
发货至: 中国

Quick Overview for CD33 抗体 (ABIN118637)

抗原

See all CD33 抗体
CD33

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD33 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

WM53
  • 特异性

    Recognizes the CD33 cell surface glycoprotein, a 67 kDa molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.

    交叉反应 (详细)

    Species reactivity (tested):Human.

    纯化方法

    Affinity Chromatography on Protein G.

    免疫原

    Human AML cells

    亚型

    IgG1
  • 应用备注

    Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections (1/50-1/100): Overnight incubation recommended. Positive control tissue: lymphatic tissue. Not suitable on Paraffin Embedded Sections. Western Blotting.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS, pH 7.4, 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    CD33

    别名

    CD33 / SIGLEC3

    背景

    CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67

    基因ID

    945

    NCBI登录号

    NP_001763

    UniProt

    P20138
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